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Apoptosis Methods and Protocols (Methods in Molecular Biology)
Rok: 2004
ISBN: 9780896038738
OKCZID: 110052106
Citace (dle ČSN ISO 690):
BRADY, Hugh J.M. Apoptosis methods and protocols. Totowa: Humana Press, 2004. xi, 304 s. Methods in molecular biology;, vol.282.
Anotace
A collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in step-by-step detail, these readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.