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Protein blotting :a practical approach
Autor: Dunbar Bonnie S.
Rok: c1994
ISBN: 9780199634378
OKCZID: 110369859
Citace (dle ČSN ISO 690):
DUNBAR, Bonnie S. Protein blotting: a practical approach. Oxford: IRL Press, c1994. xxiii, 242 s. The practical approach series, 140.
Anotace
Protein blotting techniques have become common laboratory procedures in the past few years. This text is written by scientists with expertise in these techniques. The versatility of the methods utilizing these procedures have brought about the development of different solid support matrices, as well as a wide variety of protein detection methods. While the most commonly used method in protein blotting is the use of antibodies to detect protein antigens, this technology has been expanded to examine a number of different interactions between proteins and other proteins, as well as other molecules such as carbohydrates and DNA. These methods have further been adapted for amino acid sequencing and purification of proteins for use as immunogens. This book outlines, in detail, numerous protocols and procedures which should help investigators design methods which will be optimal for their specific use.