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PCR protocols in molecular toxicology
Rok: c1998
ISBN: 9780849333446
OKCZID: 110067606
Citace (dle ČSN ISO 690):
VANDEN HEUVEL, John P., ed. PCR protocols in molecular toxicology. Boca Raton: CRC Press, c1998. 237 s. CRC Press methods in toxicology.
Anotace
Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:oQuantitative reverse transcriptase PCR and methods to examine gene expressionoDifferential display cloningoCloning and library screening by PCRoGenotype and polymorphism analysis of drug and toxicant metabolizing enzymesoBasic, non-PCR based molecular biology methodsPCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.