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In Vitro Mutagenesis Protocols (Methods in Molecular Biology)
Autor: Braman, Jeff.
Rok: 2002
ISBN: 9780896039100
OKCZID: 110417835
Citace (dle ČSN ISO 690):
BRAMAN, Jeff, ed. In vitro mutagenesis protocols. 2nd ed. Totowa: Humana Press, c2002. xvi, 287 s. Methods in molecular biology, vol. 182.
Anotace
Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today's molecular biologists reliable and powerful techniques with which to illuminate the proteome.