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Nuclear transplantation in sturgeon eggs = Jaderná transplantace u jiker jesetera | ||
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Autor: Effrosyni Fatira Anotace"The development of reproductive biotechnology is opening a new window for the conservation of threatened wildlife, as a backup when all other protection policies have failed. In this sense, nuclear transfer, also called cloning, is expected to be a useful tool to preserve species that are nearly extinct or to reconstruct extinct species. Interspecific somatic cell nuclear transfer (iSCNT) application to endangered sturgeon species has a great advantage, as the reconstruction of the critically threatened species can be achieved after a single fincell is transplanted in the egg-cytoplasmic environment of species whose eggs are easily available in farms. In the present Ph.D. study, the sterlet, considered to be a model species for sturgeon family, has been used as the egg recipient while the Russian sturgeon and the beluga, considered to be mostly favorable for caviar consumption, as well as the albino sterlet, have been used as donor fin cells. Overall, the SCNT methodology was a very delicate multi-step procedure that required optimization of many experimental conditions with precise techniques and skillful manipulations. In this study, the crucial steps of sturgeon cloning have been tested by adjustment of the experimental conditions with intraspecific and interspecific SCNT. The study demonstrated that the iSCNT can be applied to real endangered species. In addition, after the improvement of the iSCNT technique by utilizing the mSCNT, the present study could obtain for the first time a specimen (0.8%) from the donor’s origin only, while two specimens (1.6%) showed both the recipient and donor genome. These results were of high significance because the donor DNA was able to integrate into a sturgeon embryo after interspecific cloning. In all, the present Ph.D. study provides evidence that cloning with the multiple donor somatic cells can be feasible in the future of aquaculture used for meat and caviar consumption. Despite the fact that sturgeon cloning faces limitations, to date it is a promising technique for their preservation together with international protection policies that will protect the habitat of wild sturgeon population." In FATIRA, Effrosyni. Nuclear transplantation in sturgeon eggs =: Jaderná transplantace u jiker jesetera. Vodňany: Fakulta rybářství a ochrany vod, Jihočeská univerzita v Českých Budějovicích, 2020. 74 stran. ISBN 978-80-7514-104-0. Zdroj anotace: OKCZ - ANOTACE Z WEBU |
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